Skip to main content
. 2001 Nov;75(21):10372–10382. doi: 10.1128/JVI.75.21.10372-10382.2001

FIG. 5.

FIG. 5

FIG. 5

Basal and IFN-induced activation is inhibited in ASFV-infected PAECs. (A) E-Selectin (CD62E) expression on ASFV-infected PAECs. Cells were stained either with anti-E-selectin antibody 1.2B6 (solid histograms) or an irrelevant control (open histograms). (i) Resting cells, (ii) cells after infection with ASFV for 6 h, or (iii) cells stimulated with TNF-α for 6 h are shown. (B) MHC class I expression on ASFV-infected PAECs. Cells were stained with anti-MHC class I MAb 2-27-3 (solid histogram) or with an irrelevant control (open histogram). (i) Resting cells, (ii) cells infected with ASFV for 24 h, and (iii) cells stimulated with TNF-α for 24 h are shown. (C) Downregulation of IFN-α-induced MHC class I expression by ASFV in PAECs. Cells were stained with either an anti-MHC class I antibody 2-27-3 (solid histograms) or an irrelevant control (empty histograms). (i) Resting cells, (ii) cells treated with IFN-α for 24 h, (iii) cells infected with ASFV for 24 h and (iv) cells given IFN-α treatment overnight followed by ASFV infection for 4 h are shown.