FIG. 8.

PARP and NF-κB p65 cleavage in ASFV-infected PAECs: inhibition by z-VAD-fmk. Western blot analysis of lysates of PAECs infected with ASFV Malawi for 0, 4, 8, and 18 h were analyzed with anti-PARP and anti-NF-κB p65 antibodies. PARP cleavage was monitored by production of a 24-kDa fragment 4 hpi. The cleavage was inhibited by z-VAD-fmk (Z), indicating caspase-3 activation. The same lysates were probed with MAb F6 directed to the p65 subunit of NF-κB. The 65-kDa protein was present at 4 hpi, decreased at 8 hpi, and disappeared at 18 hpi. The pan-caspase inhibitor z-VAD-fmk prevented loss of the 65-kDa band at 8 hpi (Z).