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. 2024 Jun 4;116(10):1598–1611. doi: 10.1093/jnci/djae120

Figure 5.

Figure 5.

Batf knockout decreased nuclear factor–κB (NF-κB) activation. A) Quantitative polymerase chain reaction results for messenger RNA (mRNA) level of NF-κB–P65 in prostate tissues. Data are represented as mean (95% confidence interval), n = 3 animals per group, **P < .01. B) Representative prostate sections stained for NF-κB–P65 in mouse prostate tissues at 9 and 30 weeks of age. Original magnification, ×100 (scale bar, 200 µm); inserts, ×400 (scale bar, 50 µm); arrows indicate positive cell nuclear translocation. C-D) Representative Immuno blot results for NF-κB signaling in prostate cancer cell lines after exposure to conditioned media (CM) from Batf+/+ or Batf-/- mouse naive CD4+ T cells (Th0) or differentiated Th17 cells for 48 hours. E) Representative immunohistochemistry for NF-κB-p65 in LNCaP (human prostate cancer cell line) cells after exposure to CM from Batf+/+ or Batf -/- mouse naive CD4+ T cells (Th0) or differentiated Th17 cells for 48 hours. Original magnification, ×400 (scale bar, 20 µm); insert, ×800 (scale bar, 10 µm). F) Percentage of NF-κB-P65 nuclear translocation per high-power field. *P < .05, **P < .01, ***P < .001. G) Representative immunofluorescence for NF-κB–P65 in PC3 (human prostate cancer cell line) cells after exposure to CM from Batf+/+ or Batf-/- mouse naive CD4+ T cells (Th0) or differentiated Th17 cells for 48 hours. Original magnification, ×600 (scale bar, 13 µm); insert, ×1200 (scale bar, 6.6 µm). H) Percentage of NF-κB–P65 nuclear translocation per high-power field. *P < .05, **P < .01, ***P < .001.