(A) Proteins enriched (n=86, P<0.05) in the low ISHOMA group (ISHOMA<0.61) were selected for further network analysis (String V.11.5). (B) DNA replication, cell cycle and ribosome pathways were significantly enriched KEGG pathways in the low ISHOMA group. Six proteins from the MCM-complex (MCM2-7) were the drivers for enrichment of DNA replication and cell cycle pathways (Gene Ontology). (C) Clinical traits known to be dysregulated in maternal obesity in the first trimester of pregnancy were correlated with raw LFQ values (Label Free Quantification; semi-quantitative measure of concentration) of MCM2-7 proteins. All proteins of the MCM-complex, except MCM7, were positively correlated with C-peptide and inversely correlated with ISHOMA. Most individual MCM proteins were positively correlated with each other. ECM, Extracellular matrix; GO, Gene Ontology; ISHOMA, homeostatic model assessment of insulin; KEGG, Kyoto Encyclopedia of Genes and Genomes.