Table 1. Kinetic constants for a variety of purified MDH isozymes.

Organism	UniProtKB unique entry	Substrate	KM (μM)	kcat (s−1)	kcat/KM (M−1s−1)	Citation
H. sapiens (cyto*)	P40925-1	OAA	33.4 ± 0.2	200 ± 20	6 × 106	[26]
		OAA	58 ± 12			[27]
		NADH	14.3 ± 1.5	N.D.
		L-Malate	660 ± 230
		NAD+	39 ± 14
S. scrofa (cyto)	P11708	OAA	30	475	1.36 × 107	[28–30]
		OAA	35 ± 2
		OAA	8.3 ± 0.6
		NADH	21 ± 1
S. scrofa (mito)	P00346	OAA	18, 35	N.D.	N.D.	[30–32]
		NADH	38, 15
		L-Malate	370, 1100
		NAD+	230, 480
C. vulgaris (mito)	P17783	OAA	150	N.D.	N.D.	[33]
		NADH	110
		L-Malate	3690
		NAD+	460
C. vulgaris (glyox)	P19446	OAA	180	N.D.	N.D.	[33]
		NADH	130
		L-Malate	7180
		NAD+	460
A. thaliana (cyto)	P93819	OAA	238 ± 21	608 ± 22	2.6 × 106	[34]
		NADH	72 ± 7	677 ± 24	9.0 × 106
A. thaliana (chloro)	Q9SN86	OAA	324 ± 109	9.9 ± 1.4	3.1 × 104	[35]
		NADH	431 ± 28	13 ± 9	3.2 × 104
C. elegans (cyto)	Q9UAV5	OAA	54 ± 4			[36]
		NADH	61 ± 6	350 ± 21	5.7 × 106
C. elegans (mito)	O02640	OAA	52 ± 4			[36]
		NADH	107 ± 5	460 ± 23	4.3 × 106
P. falciparum	Q6VVP7	OAA	30 ± 1	960 ± 70	3.2 × 107	[25]
		NADH	36 ± 2	950 ± 80	2.6 × 107
		L-Malate	1350 ± 24	250 ± 19	1.8 × 105
		NAD+	152 ± 13	150 ± 20	9.8 × 105
E. coli	P61889	OAA	40-50	900-930	2.3 × 107	[37,38]
		NADH	61 ± 2
		L-Malate	2600 ± 200	21	8.1 × 103
		NAD+	260 ± 30
T. thermophilus	P10584	OAA	9.8	N.D.	N.D.	[39]
		NADH	6
		L-Malate	26
		NAD+	19
S. coelicolor	Q9K3J3	OAA	189	1870	1 × 104	[40]
		NADH	83	542	6.56 × 103
		L-Malate	494	4.71	9.53
		NAD+	150	3.66	24.4
H. marismortui	Q07841	OAA	130	N.D.	N.D.	[41]
		NADH	12

^*chloro, chloroplastic; cyto, cytosolic; glyox, glyoxysomal; mito, mitochondrial; N.D., not done in the published article; OAA, oxaloacetate.