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. 2024 Jul 22;70(5):320–326. doi: 10.1262/jrd.2024-039

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Fig. 3. — Distribution of H3K9me3 is altered in Kdm4d mutant mouse round spermatids. (A) Line plots showing Kdm4d (red) and Kdm4dl (blue) expression levels during spermatogenesis based on published RNA-seq data (GSE55060 and DRA000484) [24, 25]. THY, undifferentiated spermatogonia; PS, pachytene spermatocytes; RS, round spermatids; SP, sperm. (B, C) Immunohistochemistry for Kdm4d mutant mouse testis sections. H3K27me3 (B) or H3K9me3 (C) antibody was used. Scale bar, 25 μm. Signal intensity at the indicated dashed lines was measured and indicated in the right panels. Arrows in (C) indicate excessive H3K9me3 signals at the chromatin region surrounding chromocenters.