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. 2024 Jul 22;70(5):320–326. doi: 10.1262/jrd.2024-039

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©2024 Society for Reproduction and Development

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

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Fig. 4. — Nuclei of Kdm4d mutant mouse sperm can generate offspring normally. (A–D) Development of ICSI embryos generated by Kdm4d^+/− or Kdm4d^−/− mouse sperm. Wild-type eggs and Kdm4d^+/− or Kdm4d^−/− mouse sperm were used for ICSI. Implantation rate (A), birth rate (B), pups weight (C), and placenta weight (D) were analyzed. Mean ± SD are indicated. * P < 0.05 (Student’s t-test). Three independent male mice were used for each experimental group. (E) Pictures showing offspring generated by ICSI. Scale bar, 1 cm. (F) Expression of KDM4D in human tissues. KDM4D is specifically expressed in testis. Data are obtained from the GTEx portal (https://www.gtexportal.org/home/).