Table 2. ICSI/TESE-ICSI outcome using cryopreserved BN rat spermatozoa; effect of ionomycin and/or Triton X-100 treatment.
| Sperm source | Sperm suspension | Ionomycin | Triton X-100 | Injected | Survived (%) | Pronuclei-formed (%) |
Transferred | Offspring (%) |
|---|---|---|---|---|---|---|---|---|
| Epididymal | Iso-osmotic | − | − | 129 | 108 | 8 | 87 | 0 |
| (83.7 ± 5.7) | (7.4 ± 4.2) c | (0) | ||||||
| Iso-osmotic | + | − | 109 | 92 | 62 | 54 | 2 | |
| (84.4 ± 3.4) | (67.4 ± 10.0) ab | (3.7 ± 3.3) | ||||||
| Iso-osmotic | − | + | 85 | 62 | 22 | 54 | 0 | |
| (72.9 ± 4.5) | (35.5 ± 13.9) bc | (0) | ||||||
| Iso-osmotic | + | + | 80 | 69 | 65 | 61 | 4 | |
| (86.3 ± 3.8) | (94.2 ± 2.6) a | (6.5 ± 3.6) | ||||||
| Testicular | Iso-osmotic | − | − | 245 | 190 | 102 | 170 | 1 |
| (77.6 ± 6.6) | (53.7 ± 5.9) b | (0.6 ± 0.4) | ||||||
| Hypo-osmotic | − | − | 123 | 94 | 14 | 81 | 0 | |
| (76.4 ± 4.4) | (14.9 ± 5.5) c | (0) | ||||||
| Hypo-osmotic | + | − | 114 | 99 | 32 | 79 | 0 | |
| (86.8 ± 2.5) | (32.3 ± 6.3) b | (0) | ||||||
| Hypo-osmotic | − | + | 103 | 87 | 19 | 68 | 0 | |
| (84.5 ± 3.3) | (21.8 ± 3.1) bc | (0) | ||||||
| Hypo-osmotic | + | + | 101 | 86 | 51 | 56 | 0 | |
| (85.1 ± 2.3) | (59.3 ± 8.2) ab | (0) | ||||||
%: Mean ± SEM. a–c Different superscripts indicate significant differences at P < 0.05.