Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Aug 11;70(5):343–347. doi: 10.1262/jrd.2024-033

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

©2024 Society for Reproduction and Development

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

PMC Copyright notice

Fig. 2. — Isolation, cell aggregate formation, and induction of CD9-positive cells from the marginal cell layer of the intermediate lobe (IL) in the pituitary gland of adult male rats. (A) Schematic of hanging drop 3D culture using isolated CD9-positive cells and subsequent induction procedures. (B) Double immunofluorescence staining for CD9 (left panels) and SOX2 (middle panel), and a merged image with DAPI staining (right panel) in smears of CD9-positive cells. (C) Bright fields images after 2 and 3 days of hanging drop 3D culture. (D) Double immunofluorescence staining for CD9 (left panels) and SOX2 (middle panel), and a merged image with DAPI staining (right panel) of cell aggregates obtained from hanging drop 3D cell culture. PL, posterior lobe. Scale bars: 20 μm (B, C and D).