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. 2024 Oct 8;15:8718. doi: 10.1038/s41467-024-52512-1

Fig. 5. E. coli BamA lateral gate chimeras remain sensitive to PTB2 inhibition.

Fig. 5

A The lateral gate of the E. coli BamA barrel was substituted with the lateral gate sequence from A. baumannii or P. aeruginosa. As described in the main text, chimera #2 (green, right) has more extensive substitutions compared to chimera #1 (blue, left), and all swapped regions are highlighted in the context of our PTB1-1–BAM structure (left: closed lateral gate) and PTB2-BAM structure (right: open lateral gate), respectively. The approximate binding site location of PTB1-1 and PTB2 are circled with pink dashed lines. B Sequence alignments of BamA from E. coli, P. aeruginosa, and A. baumannii in the regions encompassing chimeras #1 (blue bar) and #2 (green bar) substitutions. C Minimal Inhibitory Concentrations (MICs) for BamA-binding macrocycles of PTB1-1 and PTB2-1 with E. coli strains producing the indicated BamA chimeras. MIC assays were performed in triplicate with mean values reported. Note, the more extensive chimera #2 with the A. baumannii sequence failed to grow under the assay conditions. NG no growth.