FIG. 1.

Other serotypes cannot complement the packaging defect of pm8001. (A) Restriction maps of Ad5 and pm8001. KpnI and SpeI sites are indicated by lines and arrowheads, respectively. (B and C) 293 or 293-L1 (lane 2 in both B and C) cells were infected with the indicated viruses at an MOI of 5 PFU/cell. Forty-eight hours postinfection, DNAs were extracted from purified virions and digested with KpnI and SpeI. Southern blotting was performed to determine the serotypes of the DNAs in the virions. In panel B, the arrow points to the Ad5-specific band containing the L1 gene, which is digested by SpeI in pm8001 DNA to yield the two bands indicated by the arrowheads.