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. 2024 Oct 8;14:23380. doi: 10.1038/s41598-024-74783-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — The effect of time and different loading conditions on the appearance of muscle and tendon tissue. (A) Muscle fibers in the calf muscle in different groups (FL: full loading, RL: reduced loading, ML: minimal loading) and time points, visualized by staining of elastin (red) and cell nuclei (blue). (B) Photographs of the Achilles tendon and calf muscle in different groups after 3 weeks of healing, taken from the side. (C) An intact tendon (IT) is shown as a reference. (D) Tendon diameter for different loading groups and time points, measured from histological sections. There was a significant effect of load levels during the early time-points and an effect of time between early and late healing. Scale bar 50 μm. # denotes a significant difference from minimal loading at the equivalent time point; ¤ denotes a significant difference between reduced and full loading; and ^a denotes a significant difference by time compared to 3 weeks.