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. 2024 Oct 8;14:23380. doi: 10.1038/s41598-024-74783-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Higher mean intensity for collagen 1 in unloaded tendons. (A) Immunofluorescent staining for collagen 1 (red) in healing Achilles tendons at different time points after tendon injury and exposed to different loading conditions (ML: minimal loading, RL: reduced loading, or FL: full loading). Images were taken in the middle of the tendon tissue. Magnification of the marked areas show staining of collagen 1 (red) and cell nucleus (blue). (B) Mean intensity (MI) of collagen 1 for all individual tendons (dots) and mean (black line) for each group. (C) Intact tendon (IT), shown as a reference. There was a significant effect of load levels during the early time-points and an effect of time between early and late healing. Scale bar 50 μm. # denotes a significant difference from minimal loading at the equivalent time point; and ^adenotes a significant difference by time compared to 3 weeks.