Table 3A. Schedule of events; screening and challenge periods.

	Screening period					Challenge period				Month 1								Monthly until lesion reported
			Mon	Tues	Wed	Thur	Fri	Sat	Sun	Week 1 Mon/Thu		Week 2 Mon/Thu		Week 3 Mon/Thu		Week 4 Mon/Thu		Week 1 Mon/Thu		Week 2 Mon/Thu		Week 3 Mon/Thu		Week 4 Mon/Thu
Study visit	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17
Study day		-1 to -28	0	1	2	3	4	5	6	7	10	14	17	21	24	28	31
Time window (+/- days)			0	0	0	0	1	1	1	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2	2
Eligibility check	X
Written consent		X
Verbal consent			X			X															X
History		X
Examination		X				X															X
Vital signs		X	X			X															X
Screening investigations		X
12-lead ECG		X				X
Urinary bHCG (as appropriate)		X	X			X
Laboratory safety assessment			X			X				X	X		X								X
Excipient-only challenge			X
M. ulcerans JKD8049 challenge						X
AE screening			X	X		X	X														X
Virtual volunteer diary				X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
Self-collected photograph				X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X	X
PBMC collection			X			X				X	X		X								X
Serum collection			X			X				X	X		X								X
Microbiology blood sampling						X				X	X		X								X
Skin swabs						X
GAD-7 questionnaire						X							X								X
Time (min)	20	60	300	20	20	300	20	20	20	10	10	10	10	10	10	10	60	10	10	10	10	10	10	10	60