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. 2001 Nov;75(21):10472–10478. doi: 10.1128/JVI.75.21.10472-10478.2001

FIG. 3.

FIG. 3

FIG. 3

Most cell lines permissive for HTLV-1 syncytium formation do not express Hsc70 on the cell surface. Binding of the anti-Hsc70 antibody SPA-815 (1 μg/ml) and the recombinant HTLV-1 SU derivative sRgp46-Fc (0.6 μg/ml) to target cells was monitored by flow cytometry. Binding of sRgp46-Fc (0.6 μg/ml) to cells in the presence of SPA-815 (4 μg/ml) is also shown. In each case the blue histograms represent basal fluorescence in the presence of irrelevant control sera and the red histograms represent the fluorescence profile in the presence of the detected test ligand. The data are summarized in panel B.