FIG. 1.

Coimmunoprecipitation of BPV1 L2 deletion mutants with L1. BHK21 cells were coinfected with recombinant SFV expressing BPV1 L1 and L2 deletion mutants, and 24 h postinfection, the cells were harvested and lysed. Immunopreciptation was performed with preimmune serum (not shown) and then rabbit anti-BPV1 L1 VLP (lanes 1 to 5). L2 was detected by Western blotting with monoclonal antibody C6 in anti-L1 VLP immunoprecipitates from lysates containing L1 (lanes 1 and 3 to 5) and full-length L2 (lanes 1 and 2), L2Δ2–9 (lane 3), L2Δ422–431 (lane 4), and L2Δ461–469 (lane 5).