TABLE 1.

Generation of infectious BPV1 by coexpression of BPV1 L1 and L2 deletion mutants in BPHE-1 cells^a

Capsid protein(s) overexpressed in BPHE-1 cells	Mean no. of foci	SD
L1	0	0
L2	0	0
L1 + L2	81	17.6
L1 + L2Δ2–9	0	0
L1 + L2Δ461–469	0	0
L1 + L2Δ422–431	86.3	15.3
L1 + L2mix2–9	0.3	0.6
L1 + L2mix461–469	80.3	10.5

^aBPHE-1 cells were coinfected with recombinant SFV expressing BPV1 L1 or L2 alone, or BPV1 L1 was coexpressed with L2, L2Δ2–9, L2Δ461–469, L2mix2–9, L2mix461–469, or L2Δ422–431, and 30 h postinfection, the cells were harvested and lysed in 1 ml of phosphate-buffered saline by sonication. Monolayers of C127C cells were infected by addition of lysates for 1 h at 37°C, washed, maintained in culture for 3 weeks, and stained. The mean number and standard deviation (SD) of foci per plate were calculated from three independent experiments.