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Sequences, amplicon size and annealing temperature of the primers used to amplify the Anopheles p47rec gene.
| Species | Name | Sequence 5’-3’ | Product size (bp) | Annealing temperature |
|---|---|---|---|---|
|
A albimanus
A darlingi |
681F | ATCTCGATTGGCAAACCGGT | 574 | 60 seg |
| 1260R | ATGTACAGACAACCGTGCGA | |||
| 1099F | ATCGGGCTTTAGCTGGGTTC | 922 | 90 seg | |
| 2053R | GATGGCGGGCACTATCATCA | |||
| A albimanus | 681F | ATCTCGATTGGCAAACCGGT | 899 | 90 seg |
| 1568R | GCTTCGGTATCTCCATGCCA | |||
| 1099F | ATCGGGCTTTAGCTGGGTTC | 420 | 60 seg | |
| 1568R | GCTTCGGTATCTCCATGCCA |
