Fig. 4.

Hepatocyte-derived Igκ promotes HCC progression through the modulation of lipid metabolism. A Scheme displaying the procedure used for identifying biological processes regulated by Igκ through RNA-seq and label-free LC-MS/MS proteomic analysis. B Volcano plot displaying dysregulated genes (red, upregulated genes; blue, downregulated genes) identified by RNA-seq. C The functions of differentially expressed genes were predicted via KEGG enrichment analysis. D The functions of differentially expressed proteins in biological processes were subjected via GO enrichment analysis. E Heatmap showing differentially expressed metabolism-related proteins upon Igκ knockdown in Huh7 cells. F Scheme displaying the procedure used for identifying the specific metabolic process regulated by Igκ through the untargeted metabolomics method. G OPLS-DA of metabolomics data was performed to analyse intragroup sample clustering and intergroup differences. H Volcano plot displaying dysregulated metabolites (red, upregulated metabolites; blue, downregulated metabolites). I KEGG pathway enrichment analysis revealed that glycerophospholipid and glycerolipid metabolism was the most differentially enriched pathway in HCC cells in terms of the number of differentially enriched metabolites