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. 2024 Aug 4;15(26):1889–1896. doi: 10.1111/1759-7714.15415

FIGURE 3.

FIGURE 3

MiR‐190 expression was inhibited by CBX4 transcriptionally. (a) The top four genes closely related to the miR‐190 expression from the Cancer Genome Atlas (TCGA) database. (b) The protein expression level of CBX4 in normal lung bronchial epithelial cell BEAS‐2B, lung cancer cells A549, HCC827 and H1299, detected by western blot. (c) The potential binding sites of CBX4 on miR‐190 promoter predicted by the JASPAR website. (d) ChIP assay validated the binding of CBX4 on miR‐190 in H1299 cells. (e) Dual‐luciferase reporter assay verified the regulation of CBX4 on potential binding sites of miR‐190 promoter. (f) The expression level of pre‐miR‐190 in CBX4 downregulated H1299 cells detected by real‐time quantitative polymerase chain reaction (RT‐qPCR). (g) The expression level of pre‐miR‐190 in CBX4 upregulated A549 cells detected by RT‐qPCR. *p < 0.05.