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. 2024 Aug 4;15(26):1889–1896. doi: 10.1111/1759-7714.15415

FIGURE 4.

FIGURE 4

CBX4 was a direct target of miR‐190‐3p. (a) The expression level of CBX4 in miR‐190 overexpressed H1299 cells detected by western blot. (b) The expression of miR‐190‐3p/5p verified by real‐time quantitative polymerase chain reaction (RT‐qPCR) after miR‐190‐3p/5p transfection. (c) The expression level of CBX4 in miR‐190‐3p/5p overexpressed H1299 cells detected by western blot. (d) The potential binding site of miR‐190‐3p on the CBX4 3′UTR region predicted by StarBase. (e) Dual‐luciferase reporter assay verified the regulation of miR‐190‐3p/5p on wild CBX4 3′UTR region. (f) Dual‐luciferase reporter assay verified the regulation of miR‐190‐3p/5p on the mutated CBX4 3′UTR region. (g) The expression level of CBX4 in miR‐190 downregulated A549 cells detected by western blot. *p < 0.05.