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. 2024 Jun 26;115(9):3013–3025. doi: 10.1111/cas.16258

FIGURE 3.

FIGURE 3

RECK mutants lacking binding to GPR124 failed to stimulate growth in pancreatic ductal adenocarcinoma (PDAC) cells. (A, B) Immunoblotting (IB) of the indicated proteins in the indicated cells. (C, D) Immunoblotting of soluble RECK in the conditioned media prepared from the indicated cells transduced with the indicated protein. Conditioned media were prepared by cultivating cells with serum‐free culture medium for 48 h and filtered through a 0.45‐μM filter. (E, G) Cell growth in the indicated cells transduced with the indicated protein. (F, H) Representative image of colony formation by the indicated cells transduced with the indicated protein. (I) TOP/FOP assay of PK45H cells transduced with pCXN2‐RECK, RECK mutants and pcDNA3.1 Myc‐GPR124 mixed at the ratio of 1:9 and treated with or without 10 ng/mL of WNT7A (N = 3). Control columns appear twice for the convenience of assessing significance between control and N200Q and M207W columns. *p < 0.05 against vector control; N.S., not significant.