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. 2024 Jun 26;115(9):3013–3025. doi: 10.1111/cas.16258

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© 2024 The Author(s). Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Depletion of GPR124 induces growth arrest which cannot be rescued by RECK. (A) Relative mRNA expression levels of GPR124 in PK45H cells transduced with pCXN2‐NEO or pCXN2‐RECK and then infected with lentivirus carrying shRNA targeting GPR124 or control (N = 3) (B) Cell proliferation curve of PK45H cells established in (A). (C) Representative image of colony formation by PK45H cells established in (A) (N = 3). (D) Immunoblotting (IB) of the indicated proteins in PK45H cells established in (A). *p < 0.05 against vector control; N.S., not significant.