FIG. 2.

Expression of the DC-SIGN/DC-SIGNR chimeras. (A) Equal amounts of the indicated constructs were transiently transfected into 293T cells. Two days after transfection the cells were stained with anti-AU-1 antibody and analyzed by FACS. The values represent the mean ± standard error of the mean of a single experiment that was carried out in triplicates. Comparable results were obtained in two independent experiments, though the absolute values varied. (B) Analysis of expression by Western blot. The chimeras were transiently expressed in 293T cells, and 2 days after transfection, the cells were lysed and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting with anti-AU-1 antibody. kDa, kilodaltons.