TABLE 1.
Replication property of the CA recombinants and mutants
| Clone | Single-cycle replication (%)a
|
Growth inb:
|
||||
|---|---|---|---|---|---|---|
| Virus production | Infectivity | M8166 | A3.01 | H9 | HSC-F | |
| NL432 (wt) | 100 | 100 | + | + | + | − |
| NL-SC1 | 31 ± 6 | 0 | − | − | − | ND |
| NL-SC2 | 77 ± 1 | 0 | − | − | − | ND |
| NL-SC3 | 70 ± 11 | 0 | − | − | − | ND |
| NL-CAd1 | 57 ± 2 | 0 | − | − | − | ND |
| NL-CAd2 | 58 ± 5 | 0 | − | − | − | ND |
| NL-CAi1 | 59 ± 7 | 0 | − | − | − | ND |
| NL-CAi2 | 97 ± 8 | 122 ± 4 | + | +D | +D | − |
a
293T cells were transfected with the proviral clones indicated (20 μg of each), and at 48 h posttransfection virus production in the culture supernatants and virus infectivity of the supernatants were monitored by RT (31) and MAGI (17) assays, respectively. Titers relative to that of the wt clone are shown.
b
The cells were infected with equivalent RT units of cell-free viruses prepared from transfected 293T cells, and virus growth was monitored by RT production in the culture supernatants for 3 weeks. −, no virus growth; +, virus growth; +D, virus growth with delayed kinetics; ND, not done.