Fig. 4. The expression of LINE1 in T cells plays a regulatory role in processes related to protein synthesis and cell cycle.

(A) Diagram illustrating the workflow used for identifying commonly altered pathways in RNA-seq datasets, comparing neonatal versus adult naïve CD4⁺ T cells and adult naïve CD4⁺ T cells depleted of LINE1 (LINE1 ASOs) versus control cells (scr ASOs): (1) GSEA was conducted on “Curated Reactome.” (2) Identification of gene sets that were either up-regulated or down-regulated. (3) Determination of LINE1-regulated gene sets as those shared between the two comparisons. (4) Identification of shared enriched genes within each gene set identified in (3) and relative similarity analysis. (B) Jaccard coefficients were calculated for core-enriched genes within commonly up-regulated gene sets from the two GSEA comparisons. These coefficients were clustered to assess cross-similarity among gene sets, highlighting that most of these gene sets are associated with molecular pathways related to mRNA translation and cell cycle progression.