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. 2024 Oct 9;10(41):eado2134. doi: 10.1126/sciadv.ado2134

Fig. 6. LINE1 are overexpressed in T cells during aging, reducing the rate of protein synthesis.

Fig. 6.

(A) Left, LINE1 expression by RT-qPCR in naïve CD4+ T cells isolated from individuals across various age groups: neonates, 0 to 2 years old, 3 to 6 years old, 7 to 13 years old, adults (<65 years old), and elderly (>80 years old). Each data point corresponds to an individual (n = 48). ***P < 0.001, F = 25.6, one-way ANOVA. Right, LINE1 expression by RT-qPCR in memory CD4+ T cells isolated from adult or elderly individuals (n = 11). **P = 0.002, unpaired two-tailed t test. (B) Percentage of puromycin-positive naïve or memory CD4+ T cells isolated from adult or elderly individuals (n = 3). Naïve adult versus elderly: *P = 0.02; memory adult versus elderly: *P = 0.02, unpaired two-tailed t test. (C) Schematic representation of the strategy of LINE1 knockdown with ASOs in elderly naïve and memory CD4+ T cells. (D) Percentage of puromycin-positive naïve or memory CD4+ T cells treated with LINE1 or scr ASOs and activated for 24 hours (naïve, n = 3; memory, n = 1). *P = 0.03, paired one-tailed t test. All data represent mean ± SEM; n refers to individuals.