Figure 4: Senataxin helicase activity is required for RAG DSB repair.

(A) Western blot analysis of cell lysates from proliferating or imatinib (imat.)-treated Setx^−/−, Setx^3HA/3HA and Setx^{K1945R/K1945R} and Setx^{L2131W/L2131W} abl pre-B cells using HA and KAP1 antibodies. (B) Southern blot analysis of EcoRV-digested genomic DNA from cells described in (A) with pMX-DEL^SJ and treated with imatinib (imat.) in the presence or absence of the DNA-PKcs kinase inhibitor NU7441 for the indicated times.