Figure 7: ATM activity is required for RAG DSB repair in Recql5^−/−: Hltf^−/− abl pre-B cells.

(A) Flow cytometric analysis for GFP expression from pMG-INV in WT, Setx^−/− and three independently isolated Recql5^−/−: Hltf^−/− abl pre-B cell lines treated with imatinib (imat.) in the presence or absence of the DNA-PKcs kinase inhibitor NU7441 or the ATM kinase inhibitor KU55933 for the indicated times. The percentages of GFP⁺ cells are indicated in the top left corners of the histograms. (B) Southern blot analysis of EcoRV-digested genomic DNA from cells described in (A) with pMX-DE^SJ and treated with imatinib (imat.) in the presence or absence of KU55933 for the indicated times.