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[Preprint]. 2024 Sep 23:2024.09.21.614236. [Version 1] doi: 10.1101/2024.09.21.614236

Figure 1. Early Enrichment Corrects Deficits in Reversal Learning Observed in Adult LB Mice.

Figure 1.

A. Experimental timeline. Acquisition D-I. Reversal learning (RL) J-O. B. Toys and objects used for postnatal enrichment. C. images of CTL, LB and LBT cages at P15. D. Acquisition (latency) days 1–4. rmANOVA, days: F(2.4, 204.4)= 214, P< 0.001, rearing: F(2,84)= 0.45, P= 0.81, sex: F(1,84)= 0.52, P= 0.47, interaction: F(2,84)= 0.17, P= 0.84. E. Acquisition (Learning Index) days 1–4. ANOVA, rearing F (2, 84) = 1.50, P=0.23, sex F (1, 84) = 9.55, P= 0.0027, interaction: F (2, 84) = 0.14, P=0.86. F. Acquisition (probe trial) latency to escape. ANOVA, rearing F (2, 84) = 0.62, P= 0.53, sex: F (1, 84) = 8.92, P= 0.0037, interaction: F (2, 84) = 0.008, P= 0.99.G. Acquisition (probe trial) time in target zone. ANOVA, rearing F (2, 84) = 0.46, P= 0.62, sex: F (1, 84) = 2.91, P= 0.092, interaction: F (2, 84) = 0.34, P= 0.71. H. Schematic illustration of maze during acquisition phase. I. Representative heat maps of search strategy during acquisition probe trial. J. Reversal learning (latency) days 1–4. rmANOVA, days: F(2.35, 195.17)= 164.21, P< 0.001, rearing: F(2,83)= 20.62, P< 0.001, sex: F(1,83)= 0.27, P= 0.60, interaction: F(2,83)= 0.12, P= 088. Post-hoc. CTL vs. LB: P< 0.001, LBT vs. LB: P< 0.001, CTL vs. LBT: P= 0.58. K. Reversal learning (Learning Index) days 1–4. ANOVA, rearing F (2, 84) = 18.40, P< 0.000, sex: F (1, 84) = 0.67, P= 0.41, interaction: F (2, 84) = 0.21, P= 0.81. Post-hoc. CTL vs. LB: P <0.0001, LBT vs. LB: P= 0.0004, CTL vs. LBT: P= 0.69. L. Reversal learning (probe trial) latency to escape. ANOVA, rearing F (2, 84) = 35.86, P< 0.0001, sex: F (1, 84) = 1.75, P= 0.18, interaction: F (2, 84) = 0.95, P= 0.39. Post-hoc. CTL vs. LB: P <0.0001, LBT vs. LB: P <0.0001, CTL vs. LBT: P= 0.44. M. Reversal learning (probe trial) time in target zone. Rearing F (2, 84) = 50.20 P<0.0001, sex: F (1, 84) = 1.047 P=0.30, interaction F (2, 84) = 1.536 P=0.22. Post-hoc Sidak. CTL-LB P <0.0001, LBT-LB P <0.0001, CTL-LBT P= 0.2607. N. Schematic illustration of maze during reversal learning. O. Representative heat maps of search strategy during reversal learning probe trial. CTL males n=18, CTL females n= 18, LB males n= 18, LB females n=18, LBT males n=10, LBT females n=10. From 7–9 different litters per group.