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Sequence of most mutations constructed for this study. PBS, tRNALys3 (primer) binding site; AUG, initiation codon of the Pr55gag polyprotein. Numbering is in reference to the genomic RNA cap site. The secondary structure of stem I, stem B, the klh, the SD hairpin, and SL3 is shown in Fig. 1. ΔSD maintains a wild-type-consensus CUGGUGAR splice site sequence.
