TABLE 1.
Genomic RNA dimerization yield and infectious titers of 26 HIV-1 mutantsa
| Mutation location and sequence | % Dimerization C ± SD | Log BH10/mutant ratio ± SEM |
|---|---|---|
| Within KLD | ||
| Δ247 | 54 ± 2.5 | 1.6 ± 0.45 |
| Δ271–273 | 51.5 ± 3 | 2.0 ± 0.35 |
| Δ243–246 | 48 ± 4.5 | 3.35 ± 0.20 |
| Δ274–277 | 52.5 ± 2 | 3.5 ± 0.20 |
| BH10 | 84 ± 3 | 0.0 |
| G247C | 62.5 ± 3 | 2.5 ± 0.15 |
| MB1 | 38 ± 2.5 | 3.2 ± 0.15 |
| MB2 | 55 ± 1.5 | 2.4 ± 0.1 |
| MB3 | 61 ± 6 | 2.65 ± 0.25 |
| MB4 | 64 ± 2.5 | 2.2 ± 0.25 |
| MB6 | 85 ± 1 | 3.2 ± 0.15 |
| MB7 | 73.5 ± 7.5 | 2.5 ± 0.2 |
| MB8 | 70 ± 5 | 1.6 ± 0.1 |
| MB9 | 85 ± 2 | 0.2 ± 0.25 |
| MB10 | 86.5 ± 2 | 0.3 ± 0.2 |
| M5′B9 | 69 ± 4 | 3.5 ± 0.2 |
| M5′B10 | 63.5 ± 3 | 3.6 ± 0.1 |
| 5′ or 3′ of KLD | ||
| Δ200–226 | 63 ± 3.5 | 3.5 ± 0.65 |
| M5′I-1 | 64 ± 2 | 3.3 ± 0.35 |
| MI-1 | 63 ± 5 | 3.1 ± 0.45 |
| M5′I-2 | 67.5 ± 8.5 | 3.5 ± 0.3 |
| Δ236–242 | 55 ± 3 | >4.2 |
| ΔSD | 76 ± 2 | >4.3 |
| Δ4AU | 73 ± 5.5 | 1.2 ± 0.15 |
| ΔP1 | 56 ± 3.5 | NDb |
| C258GΔP1 | 55.5 ± 2 | ND |
| ΔH3 | 73 ± 2.5 | 1.5 ± 0.15 |
a
In each infectivity test, the number of 50% tissue culture infective doses per ng of CAp24 was determined in octuplicate (14) by using a fourfold serial dilution against a control BH10 population. The number of independent infectivity tests per mutant was 2 to 4.
b
ND, not done.