Skip to main content
[Preprint]. 2024 Sep 24:2024.09.24.611806. [Version 1] doi: 10.1101/2024.09.24.611806

FIGURE 2. Transcriptional changes in neurons of cortex after sleep deprivation.

FIGURE 2.

(A) Volcano plot showing changes in gene expression for Sleep Deprived (SD)/Normal Sleep (NS) comparisons. Unpaired t-tests followed by false discovery rate (FDR) analysis were used to compare groups. Significantly downregulated (log2(SD/NS) < −0.4, FDR < 0.05) and upregulated (log2(SD/NS) > 0.4, FDR < 0.05) gene expression is color-coded with blue and red, respectively. Genes in grey are not significantly changed after sleep deprivation. (B) Heatmap showing differentially expressed genes between NS and SD conditions. (C, E) Dot plots showing significantly enriched top downregulated (C) and upregulated (E) biological processes. Circle size is proportional to gene count and the level of significance (Benjamini-Hochberg adjusted P value < 0.05) is color-coded. (D, F) Cnet plots show significantly enriched top-downregulated (D) and upregulated (F) biological processes and their gene linkages. Circle size is proportional to gene counts for biological processes. The edge is color-coded as a biological process. The fold change of downregulated and upregulated genes is color-coded with blue and red gradients, respectively. (G, H) Bar plots showing significantly enriched top downregulated (G) and upregulated (H) KEGG pathways. The level of significance (Benjamini-Hochberg adjusted P value < 0.05) is color-coded.