Figure 2. Three NPFs colocalize with Arp2/3 at different cellular and subcellular locations.
(A) Co-visualization of Arp2/3 with WAVE, WASP, and WASH in gastrulation stage embryos using endogenously tagged alleles. White arrowheads point to Ea and Ep cells. Scale bar: 5 μm. (B) Quantification of colocalization between Arp2/3 and NPFs, with box plots reporting Pearson correlation coefficients at Ep/P4 contacts, cell-cell contacts, and the cytoplasm. P values were calculated with a Dunn’s test. (center line, median; box, interquartile range (IQR); whiskers, min/max range; n ≥ 10 embryos; *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001)