Figure 3. RNAi against each NPF leads to Arp2/3 reduction at different cellular and subcellular locations.

(A) Micrographs from time-lapse movies depicting localization of Arp2/3 in control and WAVE, WASP, or WASH RNAi-treated embryos from a lateral view. White arrowheads point to Ea and Ep cells. The diagrams underneath each micrograph highlight the observed Arp2/3 localization in E and neighboring cells. (B) Violin plots reporting changes in Arp2/3 localization at Ep/P₄ contacts, cell-cell contacts, and the cytoplasm upon RNAi depletion of WAVE, WASP, and WASH. (center dot, mean; vertical line, s.d.; outline, the distribution of the data; n ≥ 10 embryos) (C) Violin plots reporting changes in the differences between the maximum and minimum gray values of the Arp2/3 signal along the line scan upon RNAi depletion of WAVE, WASP, and WASH. (center dot, mean; vertical line, s.d.; outline, the distribution of the data; n ≥ 10 embryos) All measurements were collected 6 minutes after the division of neighboring mesoderm precursor cells (MSx). P-values reported in Figures B and C were calculated using either an unpaired t-test or an unpaired t-test with Welch’s correction, depending on whether the variances between groups were equal. (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001) Scale bar: 5 μm.