Figure 6. Newly established ectopic cell-cell contact acts as a symmetry-breaking cue to recruit WAVE and Arp2/3.

(A) Micrographs from time-lapse movies of chimeric embryos created by combining two embryos expressing WAVE and Arp2/3. The DIC channel of the whole chimera is shown on the left, and two fluorescence channels of a blow-up of the chimeric contact (outlined region) are shown on the right. Yellow arrows point to ectopic cell-cell contacts. (B) Violin plots reporting normalized fluorescence intensity of WAVE and Arp2/3 at ectopic/endogenous cell-cell contacts and the contact-free cell apex. (center dot, mean; vertical line, s.d.; outline, the distribution of the data; n = 15 chimeras) P values were calculated with a Tukey test. ( *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001) Scale bar: 5 μm.