Figure 5. TgGSK knockdown parasites have a centrosome duplication defect.

A. IFA TATi-GSK parasites treated with aTC for 96 hours stained for centrin 1 (centrosomes) and EB1 mitotic spindles). Outlined boxes show parasites with abnormally dividing centrosomes. B. Quantification of centrin and EB1 signal in (A) measuring distribution of signal per parasite nucleus. N= 3 replicates with 100 vacuoles quantified. Statistical analysis: two-tailed t-test with Welsh’s correction. *: p<0.05 ns: no significance. C. Expansion microscopy of TATi-GSK parasites treated with aTC for 42 hours. Arrows indicate elongated centrosomes that seem unable to undergo fission.