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A. IFA of non-dividing GSK.3xHA parasites treated with 0, 2, or 4 μM Garcinol for 18 hours. Staining was done for IMC3, HA, and DAPI to visualize IMC, TgGSK, and nuclear material, respectively. B. Western blot analysis of TgGSK protein levels after 18 hours of Garcinol treatment. The cytosolic protein aldolase was used as a control.
