Extended Data Figure 5: Membrane insertion of the finger loop affects the middle loop’s orientation.

(a) MD simulations comparing the orientation of the middle loop of membrane-anchored β-arrestin 1 vs β-arrestin 1 in solution. The flexibility of the middle loop was illustrated using structural snapshots from accumulated frames (one snapshot every 30 ns) for β-arrestin 1 embedded in membrane (green) and β-arrestin 1 in solution (red). (b) Recruitment of FlAsH 2 biosensors for WT β-arrestins and finger loop deletion mutants (ΔFL) to the PM using the NanoBiT assay. Data shown represents mean ± SEM, n=4 independent biological replicates.