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Insertion sites of tetracysteine motif in FlAsH reporters
| Probes | FlAsH Insertion Sites | |
|---|---|---|
| β-arrestin1 | β-arrestin2 | |
| FlAsH1 | G39 | G40 |
| FlAsH2 | K138 | K139 |
| FlAsH3 | K170 | K171 |
| FlAsH4 | N223 | N225 |
| FlAsH5 | T261 | T263 |
| FlAsH6 | G409 | G410 |
Tetracysteine motif (CCPGCC) was inserted after the indicated amino acid residues of SmBiT-β-arrestin 1 and SmBiT-β-arrestin 2. FlAsH probes 1–3 are located in the N-domain, while FlAsH probes 4–6 are inserted in the C-domain.
