Figure-2.

(A) RT-qPCR analysis of total RNA from control (N=3) and Elavl1 conditional knockout limbs (N=3) from 17.5 dpc embryos indicating significant downregulation of Elavl1 RNA levels in limbs upon Prx1-cre mediated deletion. Representative whole-body images of 13.5 dpc embryos (B), neonatal mice pups of P1 stages (C) of control (Elavl1^fl/fl, Prx1-cre; Elavl1^fl/+, N=8), Elavl1 conditional knockout (Prx1-cre; Elavl1^fl/fl, N=7) showing no gross morphological differences during pre- and postnatal development. Whole body images of 4-week-old male control (N=3) and Elavl1 cKO (N=3) mice showing no gross morphological differences including limbs (D). Fore- and hind limb lengths in the proximal-distal axis in four-week-old male mice measured between control (n=3) and Prx1-cre; Ealvl1^fl/fl mice(n=3), ns: not significant