Figure 2. Lipid associated macrophage marker genes are increased in obese tumor-adjacent mammary adipose tissue (mAT^Tum-adj) and positively correlate with tumor mass.

A) Schematic for magnetically sorting CD11b⁺ cells from E0771 tumors using Miltenyi Octomacs. B) Trem2, Cd36, and Cd9 relative gene expression by RT-PCR in tumor CD11b⁺ fraction. C) Anatomical schematic of subcutaneous mAT^Tum-adj and contralateral mammary adipose tissue (mAT^Contra). D - F) Trem2, Plin2, Cd36 relative gene expression by RT-PCR from whole tissue lysates of mAT^Contra and mAT ^Tum-adj. G-H) Correlation analysis of tumor mass vs. Trem2 and Plin2 gene expression in mAT^Contra and mAT ^Tum-adj with linear regression fit. Figures D-F, solid bar = mAT^Contra, hashed bar = mAT ^Tum_adj. One-way ANOVA tests were used to compare tumor LAM marker gene expression among groups. Two-way ANOVA with Tukey’s multiple comparison tests were used to compare groups for all mAT gene expression. Simple linear regression analysis was applied to correlation plots and the p value and coefficient of determination (r²) is displayed on each graph. All data plotted as mean ± SEM for 8-12 mice per group. *denotes diet effect, †denotes tissue effect (mAT^Contra vs. mAT ^Tum-adj), **p<0.01, ***p<0.001, ****p<0.0001; †p<0.05, †††p<0 001, ††††p<00001. Figures 2A & C were created with Biorender.com.