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[Preprint]. 2024 Sep 23:2024.09.20.614087. [Version 1] doi: 10.1101/2024.09.20.614087

Figure 2: Live cell imaging in transfected HE293 cells.

Figure 2:

A-E) HEK293 expressing HTP-TM-SNAP. Intracellular SNAP labelled with BG-JF646; extracellular HTP labelled with TMR-d12-HTL or TMR-d12-SHTL. Widefield imaging (A), zoom-ins (B, D) and line scans (C, E). F-J) HEK293 cells expressing SNAP-TM-HTP. Extracellular SNAP labelled with BG-SulfoJF646; intracellular HTP labelled with TMR-d12-HTL or TMR-d12-SHTL. Widefield (F), zoom-in (G, I) and line scans (H, J). K-O) As for A-E but staining with BG-JF549 and SiR-d12-(S)HTL. P-T) As for F-J but staining with BG-SulfoJF549 and SiR-d12-(S)HTL.