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A) Required stock solutions. B) Outlined 5-minute synthetic protocol (partly created in biorender.com). C) Structures of JF549/646-SHTL. D) LCMS traces of the reaction for TMR-d12-HTL and JF646-HTL. E) Confocal imaging of HEK293:SNAP-HTP-mGluR2 transfected cells with TMR-d12-(S)HTL (50 nM) and BG-Sulfo646 (50 nM) including line scans. F) As for E, but with JF549-SHTL, SiR-d12-SHTL and JF646-SHTL.
