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[Preprint]. 2024 Sep 28:2024.09.27.615526. [Version 1] doi: 10.1101/2024.09.27.615526

Figure 3. Malibu reports intracellular malonyl-CoA dynamics.

Figure 3.

a, Ratio change of Malibu expressed in BL21 E. coli treated with either 2 μM cerulenin (dark blue, n = 8 trials), 4 μM cerulenin (medium blue, n = 8 trials), 8 μM cerulenin (light blue, n = 9 trials), or DMSO (green, n = 9 trials; p < 0.0001, 2-way ANOVA with Dunnett’s multiple comparisons test).

b, Representative images and ratio change of Malibu fused to mScarlet (Malibu-mScarlet) expressed in HeLa cells treated with either DMSO (dark blue, n = 33 cells from 8 experiments) or cerulenin for 4 hr (50 μM, light blue, n = 44 cells from 9 experiments) normalized to mScarlet expression marker (p < 0.0001, Mann-Whitney test).

c, Ratio change of Malibu-mScarlet expressed in HeLa cells treated with either DMSO (dark blue, n = 29 cells from 6 experiments) or orlistat for 12 hr (15 μM, light blue, n = 31 cells from 6 experiments) normalized to mScarlet expression marker (p = 0.0074, Mann-Whitney test).

For all figures, dot plots show the mean ± SD. For Box-and-Whisker plots, + denotes mean, and min to max is plotted. Scale bar represents 10 μm. Only comparisons with statistical significance are indicated.