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A) We convert ROIs with human NFT point annotations to detailed segmentation masks. We do so by B) generating 400×400 pixel tiles centered on the label, C) applying color deconvolution, otsu thresholding, and morphological cleaning operations, and D) performing blob detection with center-biasing and size filtering to obtain a mask. E) We then stitch each mask into its corresponding location in the ROI via union operations.
