Table 2. Results of mNGS analysis for patients in the present study.

mNGS analysis	Results
	Case 1	Case 2	Case 3	Case 4	Case 5	Case 6
Specimen	EDTA blood	BAL	Lung nodule	Serum	Serum	Serum
Organism detected (number of sequence reads)	Rickettsia japonica (6), Torque teno midi virus 2 (5)	Rickettsia felis (1469), Streptococcus mitis (15748), Streptococcus pneumoniae (3818), Klebsiella pneumoniae (45), HHV-7 (9), Prevotella melaninogenica (14316), Neisseria mucosa (4968), Actinomyces oris (4136), Lautropia mirabilis (9897), Veillonella atypica (2178), Schaalia odontolytica (4574), Porphyromonas somerae (2085), Haemophilus parainfluenzae (2324), Gemella haemolysans (2067), Treponema medium (999), Rothia mucilaginosa (1557), Granulicatella adiacens (1202), Peptostreptococcus stomatis (1182), Parvimonas micra (955)	R. felis (12), Aspergillus fumigatus (185), CMV (87), HHV-6 (11), Torque teno virus (38), Leclercia adecarboxylata (8), Ralstonia mannitolilytica (7), Bifidobacterium breve (4), Candida parapsilosis (2), EBV (1)	None	O. tsutsugamushi (2)	Staphylococcus epidermidis (4), Acinetobacter baumannii (4)

Abbreviation: mNGS, metagenomic next-generation sequencing; EDTA, Ethylene diamine tetraacetic acid; BAL, bronchoalveolar lavage fluid; HHV, human herpes virus; CMV, cytomegalovirus; EBV, Epstein-Barr virus.