Figure 2. Split fluorescent protein labeling can be spatially restricted by transgenic expression of mNG2_1–10.

A–F. Transgenic embryos expressing mNG2_1–10 under control of the fezf2 (A–B), myl7 (C–D), or ubb (E–F) promoters and injected with mNG2₁₁-H2B mRNA. A′ shows the boxed region in A with brightness rescaled to demonstrate fluorescence is localized to nuclei. G–L. Transgenic embryos expressing GFP under control of the fez1 (G–H), myl7 (I–J), or ubb (K–L) promoters. Images were acquired at 24 hours post-fertilization. Fluorescence images are maximum projections of confocal z-stacks. Scale bars in B, F, H, and L, 200 μm. Scale bars in D, J, 50 μm.