Figure 5. Directing protein localization with split-mNG2.

A. Schematic illustrating use of the split-mNG2 system to sequester proteins of interest on mitochondria. B–G. Representative images of krt8-mNG2₁₁ embryos injected with mNG2_1–10 (B–D) or mito-mNG2_1–10 (E–G) mRNA and stained with MitoTracker dye to label mitochondria. Maximum projections of confocal z-stacks. Arrows indicate colocalization between split-mNG2 and MitoTracker fluorescence. Images were acquired from the tail fin epidermis at 48 hours post-fertilization (hpf). Scale bars, 50 μm.