Fig 6. RNA sequences in the central domain of E1 enhance macrophage replication of TC83/E1_IDsyn.

(A) Schematic representation of mutant viruses constructed. RNA chimeras containing 5’ or 3’ half or the central region of E1 synonymous mutations from TC83/E1_ID-syn (B, C) Viral replication of chimeric viruses from Raw264.7 infected at MOI 0.1. Supernatants were harvested at 12 (B) or 24 (C) hpi and infectious virus titered by focus forming assay (FFA). Each experiment was performed in triplicate three times independently and the mean and SD graphed. Statistical analysis was performed using an unpaired T-test. * >0.05, ** >0.001.